Composite

Part:BBa_K1073035:Design

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)


Inducible ampR expression combined with RhlR, LasI and eforRed expression cassettes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
    Illegal NheI site found at 1119
    Illegal NheI site found at 2784
    Illegal NheI site found at 2807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2626
    Illegal BamHI site found at 1391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2188
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1866


Design Notes

no considerations.


Source

The parts for this construct were derived from the 2013 distribution kit and the iGEM Team Uppsala.

References

[Medina et al., 2003] Medina G., et al., Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol. 2003 Oct; 185(20): 5976-83. PMID: 14526008