Composite
Part:BBa_K1073035:Design
Designed by: iGEM Team Braunschweig 2013 Group: iGEM13_Braunschweig (2013-09-17)
Inducible ampR expression combined with RhlR, LasI and eforRed expression cassettes
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1096
Illegal NheI site found at 1119
Illegal NheI site found at 2784
Illegal NheI site found at 2807 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2626
Illegal BamHI site found at 1391 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2188
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1866
Design Notes
no considerations.
Source
The parts for this construct were derived from the 2013 distribution kit and the iGEM Team Uppsala.
References
[Medina et al., 2003] Medina G., et al., Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol. 2003 Oct; 185(20): 5976-83. PMID: 14526008